Functional characterization of NRAMP3 and NRAMP4 from the metal hyperaccumulator Thlaspi caerulescens
نویسندگان
چکیده
منابع مشابه
The molecular physiology of heavy metal transport in the Zn/Cd hyperaccumulator Thlaspi caerulescens.
An integrated molecular and physiological investigation of the fundamental mechanisms of heavy metal accumulation was conducted in Thlaspi caerulescens, a Zn/Cd-hyperaccumulating plant species. A heavy metal transporter cDNA, ZNT1, was cloned from T. caerulescens through functional complementation in yeast and was shown to mediate high-affinity Zn(2+) uptake as well as low-affinity Cd(2+) uptak...
متن کاملExpression and functional analysis of metal transporter genes in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens.
Zinc (Zn) hyperaccumulation is a constitutive property of Thlaspi caerulescens, whereas cadmium (Cd) hyperaccumulation varies greatly among different ecotypes. The molecular basis of this variation is unknown. Ecotypic differences in the sequences and expression of four representative ZIP family transporter genes were investigated. Genome analysis indicated the presence of at least two closely ...
متن کاملTranscription profiling of the metal-hyperaccumulator Thlaspi caerulescens (J. & C. PRESL).
Thlaspi caerulescens is a well-studied metal-hyperaccumulator of zinc, cadmium and nickel, belonging to the Brassicaceae family. Moreover it is one of the few hyperaccumulators that occur on different metalliferous soil types, as well as on nonmetalliferous soils. We are interested in the development of systems to improve phytoremediation of metal contaminated soils through improved metal-accum...
متن کاملTransport and detoxification of cadmium, copper and zinc in the Cd/Zn hyperaccumulator plant Thlaspi caerulescens
Hyperaccumulators store most of the accumulated metal in the vacuoles of large leaf epidermal cells (storage cells). For investigating cadmium uptake, we incubated a protoplast mixture obtained by digestion of leaves of Thlaspi caerulescens (Ganges ecotype) with a Cd-specific fluorescent dye. A fluorescence kinetic microscope was used for selectively measuring Cd-uptake and photosynthesis in di...
متن کاملComparative transcriptome analysis of the metal hyperaccumulator Noccaea caerulescens
The metal hyperaccumulator Noccaea caerulescens is an established model to study the adaptation of plants to metalliferous soils. Various comparators have been used in these studies. The choice of suitable comparators is important and depends on the hypothesis to be tested and methods to be used. In high-throughput analyses such as microarray, N. caerulescens has been compared to non-tolerant, ...
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ژورنال
عنوان ژورنال: New Phytologist
سال: 2008
ISSN: 0028-646X,1469-8137
DOI: 10.1111/j.1469-8137.2008.02694.x